What method is used to recover Acanthamoeba from damaged corneal tissues?

The correct method for recovering Acanthamoeba from damaged corneal tissues is through non-nutrient agar cultures seeded with E. coli. This technique is particularly effective because Acanthamoeba is a type of protozoan that can be isolated from clinical samples, such as corneal scrapings from patients suffering from keratitis due to this organism.

Non-nutrient agar provides a suitable environment for Acanthamoeba, allowing it to grow and be distinguished from other contaminants. By adding E. coli to the surface of the agar, a food source is created that can facilitate the growth of Acanthamoeba, as it can prey on the bacteria. This method is specifically utilized in the laboratory for the isolation and identification of Acanthamoeba from clinical specimens.

The other methods presented in the choices are not designed for the recovery of Acanthamoeba from corneal tissues. Staining methods like trichrome and Giemsa's stain are primarily used for visualization of various organisms under a microscope, rather than for culture recovery. Fecal immunoassays are not relevant here, as they are used for different parasites and conditions related to the gastrointestinal tract, rather than for isolating Acanthamo